Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae.
نویسندگان
چکیده
The beginning of the Saccharomyces cerevisiae HIS4 gene has been fused to the structural gene for Escherichia coli beta-galactosidase. This construction, which contains HIS4 DNA from -732 to +30 relative to the translation initiation codon, has been integrated into the yeast genome at two chromosomal locations, HIS4 and URA3. At both locations, this 762-base-pair stretch of DNA is sufficient for initiating expression of beta-galactosidase activity in S. cerevisiae and confers upon this activity the regulatory response normally found for HIS4.
منابع مشابه
Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae.
The role of cis- and trans-acting elements in the expression of HIS4 has been examined by using HIS4-lacZ fusions in which lacZ expression is dependent upon the HIS4 5' noncoding region. The cis-acting sequences involved in regulation were defined by studying the effects of the wild-type and various deletions and their revertants on regulation via the general control of amino acid biosynthesis....
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ورودعنوان ژورنال:
- Molecular and cellular biology
دوره 2 10 شماره
صفحات -
تاریخ انتشار 1982